Statement about Streptomyces venezuelae Strain and Sequence

Dear S. venezuelae researchers,

There has been some confusion, for which I apologise, over the multiple iterations of the Streptomyces venezuelae genome sequence (sve, sven_15 and vnz). To clarify, all the Streptomyces venezuelae work at the John Innes Centre has been performed with the same strain. The final and complete (vnz) genome sequence of this strain has been determined and submitted to the NCBI database under reference NZ_CP018074.1. In parallel, to unambiguously link the two, the sequenced strain has been deposited with the NRRL culture collection under stock code NRRL B-65442. Please use the NZ_CP018074.1 genome sequence and vnz gene identifiers from now on. A clarifying statement like this one has been added to the beginning of the Material and Methods section of the bldC paper by Bush et al. [mBio (2019) e02812-18], which can be cited in reference. Please note that Streptomyces venezuelae NRRL B-65442 carries a 158-kb linear plasmid (vnz pvnz here in StrepDB; NCBI reference NZ_CP018075.1) and this was not assembled in the first iteration of the genome sequence (sve).

In the NRRL culture collection database, you will see that it says that the origin of NRRL B-65442 is "unsure". This is only because we cannot unambiguously trace the complete lineage/history of our strain back to the 1940s. However, its genome sequence is more than 99.999% identical to ATCC10712, and so it is clearly descended from the Caracas/Yale/Burkholder strain (Ehrlich et al 1947. Science 106: 417; Ehrlich et al. 1948. J Bacteriol 56: 467-477) and not from the Illinois Agricultural Experiment Station at Urbana/Gottlieb strain (Gottlieb et al. 1948. J Bacteriol 55: 409-17). If you wish to work with our strain it can be obtained directly from the NRRL culture collection (stock code NRRL B-65442) or from us. If you have obtained S. venezuelae from the John Innes Centre, then you already have NRRL B-65442.

Prof. Mark Buttner
Department of Molecular Microbiology
John Innes Centre
Norwich Research Park
Norwich NR4 7UH
United Kingdom